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IMViC TEST
Written on: Tuesday, July 28, 2009
Time: 9:46 AM
The IMViC test
 The Indole test · Used to determine the ability of an organism to cleave the amino acid typtophan into indole, ammonia, and pyruvic acid. · Procedures involves inoculation of a medium containing the amino acid tryptophan · If organisms has the enzyme tryptophanase, removal of the amino group from tryptophan forms indole · Indole is easily detected by the addition of Kovacs’reagents and the appearance of a cherry-red-coloured layer 
The Methyl Red Test
· Used to differentiate two major types of facultatively anaerobic enteric bacterial species
· These organisms first metabolise glucose aerobically thus, exhausting all of the available oxygen by means of respiratory metabolism
· The type of fermentations and the end products formed are of value in species identification
 Voges-Proskauer · Used to detect acetoin · Is an intermediate compound produced by organisms carrying out butylene glycol type of glucose fermentation · Positive reaction àformation of pink or cherry-red colour upon the addition of alpha naphthol and potassium hydroxide creatine solutions
Citrate test
· Determines the presence of enzyme that enable citrate to enter the cells and be used as a sole source of carbon for metabolism and growth
· Media used for citrate testàSimmons’ citrate and Koser citrate
· Simmons’ citrate agar contain the pH indicator bromothymol blue, which is green under acidic conditions and dark blue when medium becomes alkaline
· Organism utilising citrate produce an alkaline reactions
· Positive reaction in Koser citrate medium is indicated by the preparation becoming cloudy after incubation
· A light inoculum must be used with Koser citrate to avoid false positive results
|
IMViC TEST
Written on: Tuesday, July 28, 2009
Time: 9:46 AM
The IMViC test
The Indole test · Used to determine the ability of an organism to cleave the amino acid typtophan into indole, ammonia, and pyruvic acid. · Procedures involves inoculation of a medium containing the amino acid tryptophan · If organisms has the enzyme tryptophanase, removal of the amino group from tryptophan forms indole · Indole is easily detected by the addition of Kovacs’reagents and the appearance of a cherry-red-coloured layer
The Methyl Red Test
· Used to differentiate two major types of facultatively anaerobic enteric bacterial species
· These organisms first metabolise glucose aerobically thus, exhausting all of the available oxygen by means of respiratory metabolism
· The type of fermentations and the end products formed are of value in species identification
Voges-Proskauer · Used to detect acetoin · Is an intermediate compound produced by organisms carrying out butylene glycol type of glucose fermentation · Positive reaction àformation of pink or cherry-red colour upon the addition of alpha naphthol and potassium hydroxide creatine solutions
Citrate test
· Determines the presence of enzyme that enable citrate to enter the cells and be used as a sole source of carbon for metabolism and growth
· Media used for citrate testàSimmons’ citrate and Koser citrate
· Simmons’ citrate agar contain the pH indicator bromothymol blue, which is green under acidic conditions and dark blue when medium becomes alkaline
· Organism utilising citrate produce an alkaline reactions
· Positive reaction in Koser citrate medium is indicated by the preparation becoming cloudy after incubation
· A light inoculum must be used with Koser citrate to avoid false positive results
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